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Journal: Cureus
Article Title: Machine Learning Analysis of Type VII Secretion System Expression and Its Relationships With Virulence Traits and Antibiotic Resistance in Staphylococcus aureus
doi: 10.7759/cureus.104870
Figure Lengend Snippet: (a) PCR results: L – 100 bp ladder; lanes 1–7 – samples showing a positive 291 bp amplified PCR product; NC – negative control (ATCC E. coli 25922); PC – positive control ( S. aureus 16S rRNA gene, 257 bp). (b) qPCR results: L – 100 bp ladder; lanes 1–11 – samples displaying a positive 196 bp amplified qPCR product; PC – positive control ( S. aureus 16S rRNA gene, 257 bp); NC – no-template control. (c) Scatterplot showing esxA gene expression across samples, with Ct values ranging from 18 to 30. (d) Grouping of isolates by sample type demonstrating uniform T7SS expression across categories. Analysis was performed using Python v3.9. PCR: polymerase chain reaction; qPCR: quantitative polymerase chain reaction.
Article Snippet:
Techniques: Amplification, Negative Control, Positive Control, Control, Gene Expression, Expressing, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction